A series of vampire killings in Nagasaki lead Asato Tsuzuki to a confrontational encounter with his new partner, Hisoka Kurosaki. As the two try to cope with working together as guardians of death they must also figure out who is responsible for the supernatural attacks.

Maria Won is revealed to be the attacker responsible for the Nagasaki attacks, however her life after death was not without the helping hand of another. As it becomes clear that the mysterious Dr Muraki is involved in the supernatural goings-on, Tsuzuki learns that Maria Wong is not the only one he should be concerned about.

Tsuzuki rushes to the aid of Hisoka, who has been captured by Dr Muraki, however during the confrontation he discovers the shocking truth behind Hisoka's death. As the two partners are reunited they are thrown into working together as Muraki attacks, trying to force the pair into submission and preventing them from escaping.

Hijiri Minase's joy at being able to play the violin again thanks to a donated cornea fades as his precious instrument is destroyed in a fire. Offered a replacement by Kazusa, the daughter of the cornea donor, little does Hijiri realise the secrets that the violin holds or that the true despair is only just beginning.

Hijiri is crushed to learn that by receiving Tatsuya Otonashi's cornea he has been entered into a contract with the devil that will culminate in the death of both Hijiri and his close friend, and Tatsuya's daughter, Kazusa. Determined to defy the pact, Hijiri tries to go on with life as normal but soon discovers this is not an option and that the assistance of Tsuzuki and Hisoka may be his only solution.

Despite what he may believe, Hijiri Minase's contract with the devil is far from over and now Tsuzuki, under the devil's control, is looking to fulfill both parts of the contract. Distraught at his friend and partner's suffering, Hisoka set a plan in motion with the hopes of freeing Tsuzuki from Sagatanas' control, however the risks are great for all involved.