Born in Cincinnati, Ohio, Nikki Glaser began performing stand-up comedy after encouragement from friends while enrolled at the University of Kansas. While still in school, she spent as much time as she could on stage. Just before graduating with a degree in English, Glaser auditioned for Season 4 of "Last Comic Standing" (NBC 2003-) in Chicago and moved on to the semi-finals in Los Angeles. She didn't make it to the finals, but made such an impression on the show that she moved to Los Angeles to focus on a career in comedy. As she continued to make stand-up appearances across the country, Glaser teamed with comedian and writer Sara Schaefer to create the podcast "You Had To Be There," offering interviews and insights from the two hosts. They put out the podcast from 2011 until early 2013, when MTV decided that they would be ideal co-hosts of their first late night talk show since "The Jon Stewart Show" (MTV, 1993-95). "Nikki & Sara Live!" (MTV, 2013) ran for two seasons on the network before it was canceled. Glaser also made a number of appearances with Amy Schumer on her sketch show "Inside Amy Schumer" (Comedy Central, 2013-), and appeared in Schumer's first film as screenwriter and star, "Trainwreck" (2015), directed by Judd Apatow. In 2015, it was announced that Glaser would star in her own talk series, "Not Safe with Nikki Glaser" (Comedy Central 2016).