Dr. Baldev Chaddha (Annu Kapoor), a well-qualified fertility expert, runs a fertility clinic and a sperm bank in Daryaganj, New Delhi, which guarantees high quality and specialized sperm for each client. Unfortunately, he has more failed cases to his credit than successful ones. A healthy, high performing donor is the need of the hour. Vicky Arora (Ayushmann Khurana), a young, good-looking, Punjabi boy from Lajpat Nagar, is the financially draining only son of Dolly, a widow, who runs a small beauty parlor from home. As luck would have it, a small brawl in the colony brings Dr. Chaddha and Vicky face to face, and Chaddha concludes that Vicky could be just the donor he has been looking for. From here on, Chaddha’s days and nights are spent convincing Vicky to become a donor, until he finally gives in. Vicky finds his love interest in Ashima Roy (Yaami Gautam), a pretty Bengali girl, who works in a bank. But soon, their hunky-dory world comes crashing down because of Vicky's past as a donor. A funny and emotional roller coaster ride, Vicky Donor is a light-hearted take on the subject of sperm donation.