A young shinobi named Asuka carries out a daring mission. The following day, she returns to Hanzo Academy in Asakusa, Tokyo, where she secretly trains with four other classmates. Their afternoon field work holds an unexpected surprise in store for them.

Asuka and the others continue training, but are fobbidden from any contact with those outside the school. Asuka feels disappointed when everyone but herself is able to perform the ninja animal-summoning technique. Asuka's Grandpa Hanzo visits the school.

Ikaruga and Asuka are confronted by an overpowering Yomi outside the supermarket. Following the confrontation, Ikaruga is left with doubts about her abilities. Meanwhile, Ikaruga's adpoted brother turns up missing. Asuka faces her fear of frogs.

The class members travel to Shinobi Island, a training ground used by shinobi for centuries. Upon their arrival, they are tasked with cleaning the boarding house, where Hibari discovers the written aspiration of a past student to become a "Super-ninja."

The Hanzo girls train even harder to be ready for the Hebijo shinobi's next move. The Hebijo girls sneak onto campus to locate the Hanzo girls' training facility. Upon finding the old schoolhouse, it is placed in a kekkai, trapping the Hanzo girls inside!

The Hanzo shinobi continue to fight one-on-one against the Hebijo shinobi. When Yagyu manages to defeat her opponent and free herself from her pocket of kekkai space, she attempts to enter Hibari's pocket to protect her, at great physical risk to herself.