A group of beautiful girls learn the ancient martial art of ninjutsu at their hidden academy; young shinobi Asuka is sent on a daring mission.

Asuka and the others continue training, but are fobbidden from any contact with those outside the school. Asuka feels disappointed when everyone but herself is able to perform the ninja animal-summoning technique. Asuka's Grandpa Hanzo visits the school.

Ikaruga and Asuka are confronted by an overpowering Yomi; Ikaruga's adpoted brother turns up missing; Asuka faces her fear of frogs.

The class members travel to Shinobi Island; Hibari discovers the written aspiration of a past student to become a Super-ninja.

The Hanzo girls train even harder to be ready for the Hebijo shinobi's next move.

The Hanzo shinobi continue to fight one-on-one against the Hebijo shinobi.