Two years after a momentous cattle drive under the guidance of cowboy Curly (Jack Palance) in the first City Slickers, Curly has passed away, and Mitch (Billy Crystal) discovers the dead man's treasure map, which shows the location of a hidden cave containing the stash. With his brother Glen (Jon Lovitz) and best friend Phil (Daniel Stern), Mitch sets out to find Curly's gold. On the trail, the trio once again battle danger, this time with the help of Curly's twin brother, Duke (Palance).