The Yagyu clan is tasked with bringing order to the Tokkaido Highway. Matajuro becomes a secret officer, while Jubei is known as the one-eyed demon.

Matajuro uncovers a deeper vendetta between a mysterious assassin and a local minister during a princess's bridal procession.

In Hakone, Matajuro helps a newlywed couple ensnared by smugglers and their cruel practices.

In Numazu, bridge construction is disrupted by an attack, and a beautiful woman distracts everyone.

Matajuro and Jubei discover a mafia turned gun smuggler supplying firearms to a clan, threatening the country's stability.